Furthermore, in line with previous findings that ROS [40], sepsis [41], or endotoxemia [42] negatively affects ATP synthase activity, the late inhibition and depletion of ATP synthase subunit β observed in the present study 36 h after the onset of CLP may also explain mitochondrial uncoupling of ATP production from oxygen consumption. This evidence concerns the gene ATP5PB and serum lipopolysaccharide activity.