To confirm whether EV71 infection causes robust production of pro‐inflammatory cytokines, real‐time PCR and western blotting were conducted to assess the expression of IL‐1, IL‐6, TNF‐α, IFN‐β, and IFN‐α at the protein and mRNA levels after EV71 infection of human colorectal, Vero, and rhabdomyosarcoma cells. The gene discussed is IFNB1; the disease is rhabdomyosarcoma.