TQ-NLC has also been proven to exhibit cytotoxicity towards breast cancer cell lines (human estrogen receptor negative breast adenocarcinoma, MDA-MB-231, and human estrogen receptor positive breast adenocarcinoma, MCF-7) and cervical cancer cell lines (human cervical adenocarcinoma, HeLa, and human squamous carcinoma, SiHa) [29]. The gene discussed is ESR1; the disease is breast cancer.