Additional CNLs affecting known breast cancer-, hMBCs-, and EMT-related genes were only detected after prolonged subculturing; including, FCA A1 containing TERT, BRCA2, RB1, and FOXO1 genes; chromosome A2 harbouring EGFR, TWIST, MLL2, and AHR; chromosomes B2, B3, and B4 harbouring CCND3, FOXA1, and MDM2, respectively; and chromosome E1 harbouring NF1, PPM1D, and KSR1. This evidence concerns the gene NF1 and breast cancer.