Next, the IC50 values of ipatasertib in HCT116 WT and p53−/− cells were calculated by using CCK-8 (10.58 and 9.149 μmol/L, Figure S1A and S1B, Table S1A and S1B), which suggested that 10 μmol/L was an appropriate dose for inhibiting colon cancer cell proliferation and p53 played little function in this process. This evidence concerns the gene TP53 and malignant colon neoplasm.