This is further confirmed by the dose response to Ang1 in both shRNA-α5 and siRNA-Ctl-treated OGD/R cells and is consistent with experiments showing that α5β1 integrin does not directly induce Tie2 signaling, but acts to sensitize Ang1-induced Tie2 activation after cerebral ischemia. The gene discussed is TEK; the disease is brain ischemia.