The induction of α5β1 was first noticeable after 4 days post ischemia in vivo, followed by an increase in Ang1 at day 7 post ischemia1,9, which is consistent with the time course of the response to OGD/R at 24 and 48 h for α5β1 and Ang1 on BECs in vitro, respectively, indicating that the induction of Ang1 lags behind that of α5β1 after cerebral ischemia. Here, ANGPT1 is linked to brain ischemia.