We used TMA images from the population-based Carolina Breast Cancer Study Phase 3 (2008–2013) to perform deep learning-based image analysis aimed at capturing larger scale and more complex properties including tumor grade, histologic subtype, estrogen receptor (ER) status, intrinsic breast cancer subtype, and Risk of Recurrence (ROR)-PT score.2 This evidence concerns the gene ESR1 and breast carcinoma.