Several of the targets identified in the C. elegans screen had previously been implicated in CCM; these included TLR4 (Tang et al, 2017), metalloproteinases (MMP2, MMP7, MMP13, MMP14; Zhou et al, 2015), and HMGCR (Nishimura et al, 2017), which is a strong validation of the DePick method (Table EV3). This evidence concerns the gene MMP14 and cerebral cavernous malformation.