Mechanistic analysis demonstrated that miR-214 inhibition reversed the suppressive effect of DKK3 knockdown on hypoxia-induced NRK-52E cell apoptosis by inactivation of Wnt/β-catenin signaling in vitro, while miR-214 ameliorated AKI in vivo through decreasing apoptosis by targeting DKK3 and activating Wnt/β-catenin pathway. The gene discussed is DKK3; the disease is acute kidney injury.