To define the extent to which impairment of self-renewal capacity was linked to suppression of breast cancer stemlike phenotype, vMCF-7Raf-1 1GX and vMCF-7Raf-1 1GXCRISPR-NOTCH3 MPS were stained for CD44 and CD24 breast cancer stemness markers. The gene discussed is NOTCH3; the disease is breast carcinoma.