FOXP3 and neoplasm: D-TILs was here defined as numbers of CD4+ cells, with and without FOXP3, and CD8+ lymphocytes per mm2 of tumor determined through 180 multispectral, multicolor immunofluorescence (IF) image cubes (×200 magnification; median of 3 images per case; n = 51 selected CRPC biopsies).