Moreover, higher nuclear SHCBP1 was detected in NSCLC cells cultured in serum-free medium than in serum-containing medium, as well as in CD44/ EpCAM double-positive cells compared to the corresponding parental tumor cells (Fig. 5g, h), suggesting an important role of nuclear SHCBP1 in CSCs regulation. Here, CD44 is linked to non-small cell lung carcinoma.