EGF and central nervous system cancer: Interestingly, of such post-translational modifications, tyrosine phosphorylation and lysine acetylation have been found to contribute to the modulation of β-catenin signaling, as supported by a previously reported study using the U87 glioma cell line showing EGF induced tyrosine phosphorylation of nuclear β-catenin [34, 35], while our data found no change in the tyrosine phosphorylation of β-catenin in nuclear extracts of 293T and A549 cells but a remarkable enhancement of lysine acetylation in nuclear β-catenin immunoprecipitation in response to EGF stimulation (Fig. 3d).