As shown in Fig. 4a–d and Supplementary Figs. 4a, b, we found that depletion of SHCBP1 remarkably repressed the cellular stemness enhancement caused by EGF stimulation, as revealed by reduced EGF-promoted formation of tumor spheres, expression of stem cell markers, and CD44/EpCAM double-positive as well as SP fraction. Here, SHCBP1 is linked to neoplasm.