EGF and non-small cell lung carcinoma: To explore how SHCBP1 mediates EGF activation of β-catenin signaling, the effects of EGFR activity on SHCBP1/β-catenin binding were examined, and we found that EGF stimulation of EGFR in 293T or A549 cells increased SHCBP1-binding β-catenin in the nuclear extraction, while TKI treatment in HCC827 or HCC4006 NSCLC cells revealed the opposite effects (Fig. 3a, b and Supplementary Fig. 3a, b), indicating that EGFR activation indeed promotes an interaction between SHCBP1 and β-catenin.