Moreover, higher nuclear SHCBP1 was detected in NSCLC cells cultured in serum-free medium than in serum-containing medium, as well as in CD44/ EpCAM double-positive cells compared to the corresponding parental tumor cells (Fig. 5g, h), suggesting an important role of nuclear SHCBP1 in CSCs regulation. The gene discussed is SHCBP1; the disease is non-small cell lung carcinoma.