To further characterize the effect of SHCBP1 on mediating NSCLC tumorigenesis, we examined the tumorigenicity of SHCBP-overexpressing NSCLC cells at low cell numbers, and found that inoculation of as few as 5 × 102 SHCBP1-A549 cells resulted in tumor growth at week 6. This evidence concerns the gene SHCBP1 and non-small cell lung carcinoma.