To ensure that this observed glucose intolerance phenotype was not due to non-specific actions of the presence of INS2-cre recombinase expression, we compared INS2cre/+, Y1lox/+ and Y1lox/+/INS2cre/+ and found no difference in blood glucose levels, insulin sensitivity and glucose tolerance between these groups (Supplementary Fig. 3g−i). This evidence concerns the gene INS and Glucose intolerance.