TFAP4 and intestinal cancer: When we analyzed ApcMin mice with intestinal epithelial cell (IEC)-specific deletion of Ap4, which was achieved by crossing Villin-Cre with Ap4fl/fl mice, we obtained similar results as for ApcMin mice with germ-line deletion of Ap4: that is, in Ap4ΔIEC/ApcMin mice intestinal cancer-related death was significantly delayed by 110 days, with heterozygous mice showing an intermediate delay (Supplementary Fig. 2f).