We performed quantitative chromatin-immunoprecipitation (qChIP) analysis to confirm Ap4 occupancy in the murine CRC cell line CT26 at the promoters of the following genes: Ascl2, Dll1, Dll4, EphB3, Hes1, Jag1, Jag2, Notch1, Sox4 and Tcf7 (Fig. 4d). Here, NOTCH1 is linked to colorectal carcinoma.