In order to assess the independent risk factors of osteosarcoma patients, we performed multivariate Cox regression analysis including Enneking stage, tumor size, distant metastasis, histological grade and present GAPLINC expression, and found that distant metastasis and GAPLINC expression were independent risk factors of osteosarcoma patients (P<0.001 and P=0.020, respectively). This evidence concerns the gene GAPLINC and osteosarcoma.