GAPLINC and osteosarcoma: Then, we also measured GAPLINC expression in osteosarcoma cell lines (MG-63, HOS, SJSA-1, and Saos-2) and the human normal osteoblast cell line (hFOB1.19) through RT-qPCR, and found that GAPLINC expression was overexpressed in osteosarcoma cell lines (MG-63, HOS, SJSA-1, and Saos-2) compared with human normal osteoblast cell line (hFOB1.19) (P<0.001, Figure 1B).