In immunoblotting and qPCR analyses, poorly differentiated cell lines also showed more resemblance to expected tumour profile based on their expression of known targets such as CDH1, VIM, CAV1, PKM2, pERK, mitochondrial pyruvate carrier 1 (MPC1), and matrix metalloproteinase 9 (Additional file 1: Figure S1d–e). The gene discussed is VIM; the disease is neoplasm.