MIR31HG and hepatocellular carcinoma: Moreover, the colony formation capacity of HCC cells in pcDNA3.1-MIR31HG + anti-miR-575 group was reduced than that in pcDNA3.1-MIR31HG + pre-miR-575 group, whereas knockdown of MIR31HG and overexpression of miR-575 significantly increased the colony formation capacity of HCC cells (Fig. 6b).