To assess the effect of PAR1 expression on tumor cells and the suspected counterbalancing activity, cells derived from p48-CRE/LSL-KRAS/P53flox/flox mice (named KP hereafter) and Panc02 murine pancreatic cancer cells were transduced with short hairpin RNA against PAR1 (shPAR1) or with control short hairpin RNA (shCtrl). Here, F2R is linked to familial pancreatic carcinoma.