ESR2 and renal cell carcinoma: To further confirm the above in vitro cell lines data with the in vivo mouse model, we implanted human RCC 786‐O cells with or without ERβ knockdown (786‐O sh‐ERβ/sh‐Luc) and A498 cells with or without ERβ over‐expression (A498‐ERβ/Vec) orthotopically and subcutaneously in male (Fig. 6A) and female mice (Fig. 6B and C).