Its aim was to confirm whether the Rc TR extract could inhibit the glioma cells viability and induce apoptosis by increasing the number of cleaved Poly(ADP-ribose) (PARP1)-positive cells, inducing DNA damage, thus altering the level of phosphorylated H2A.X-positive cells a marker of double strand breaks in DNA. Here, PARP1 is linked to central nervous system cancer.