As phosphorylation of H2A.X at Ser139 is also observed in response to DNA double-strand breaks (Kaur et al. 2006), the present study also measured by the flow cytometry the content of γ-H2A.X-positive cells in the population of patient-derived glioma cells and U87MG cells after treatment with Rc TR extract. The gene discussed is H2AX; the disease is glioma.