To clarify the molecular mechanisms of the miR‐223/IL‐6 secretion pathway in human neutrophils after infection with S. aureus, we established a miR‐223 knockdown human promyelocytic leukemia HL‐60 cell line that could be differentiated to neutrophils upon treatment with DMSO using miR‐223 AS ODN. The gene discussed is IL6; the disease is acute promyelocytic leukemia.