Using publicly available breast cancer expression datasets profiled using frozen [11] and FFPE [10] samples with known ER and HER2 status, we evaluated the different versions of the de-novo ER/HER2 gene-modules along with the ER/HER2 gene-modules from Desmedt et al. [9] using ROC analysis (Fig 5). The gene discussed is ESR1; the disease is breast carcinoma.