Herein, we present an important screening of CACNA1A gene to identify new point mutations (by Next Generation Sequencing) and to search CNVs (using MLPA assay) in patients affected by hemiplegic migraine, episodic ataxia and, for the first time, in migraine with aura not hemiplegic (MA) and migraine without aura (MO). The gene discussed is CACNA1A; the disease is migraine disorder.