In contrary, cohort study from Hyderabad, India reported that “A” allele of TLR1-248 Ser/Asn (rs4833095 G/A) associated with TB protection and evoke a better immune response by activation of TNF-α production and transcription factor NF-kB in Mtb lysate infected peripheral blood mononuclear cells (PBMC) and human embryonic kidney (HEK) cells in vitro (85). This evidence concerns the gene TNF and tuberculosis.