NES and neoplasm: In contrast to a study by Skoda et al. [35], in which Nestin was expressed in 95% of all cells derived from primary PDAC cell lines, pointing to a common expression of this protein in the entire tumor cell population, we found Nestin to be differentially expressed in Panc1 and Panc89 para- and holoclones with Nestin being exclusively expressed in holoclone cells.