Significantly enhanced TNF-α serum cytokine level (316 ± 53 ng/ml) was noted for HIFU plus TB mice tumor groups compared to untreated control (58.3 ± 1.15 ng/ml), HIFU (84.7 ± 3.93 ng/ml) and Salmonella (110.48 ± 7.82 ng/ml) treated mice (Fig. 6a). This evidence concerns the gene TNF and tuberculosis.