ANXA5 and melanoma: To assess the lethality of vemurafenib for melanoma cell lines with the V600BRAFmutation, the ten lines were exposed to vemurafenib at the same concentration (0.1 μM, added twice weekly) for 14 days and then analyzed by flow cytometry with PE-conjugated Annexin V. As shown in Figure 2C, a significant increase of the number of annexin V-positive cells was detected in the five sensitive lines and only in one out of the five resistant cell lines (MM164).