TNF and familial pancreatic carcinoma: Using Panc-1 (p53mt), MiaPaCa-2 (p53mt), and Capan-2 (p53wt) pancreatic cancer cell lines, we found that rfhSP-D treatment for 24 h caused growth arrest in G1 cell cycle phase and triggered upregulation of pro-apoptotic markers, TNF-α, and Fas, which eventually caused apoptosis by 48 h (29).