Furthermore, although the levels of key Hedgehog signaling members GLI1 and GLI2 were decreased in CRC compared with the normal tissues (Supplementary Fig. 15B), they were increased in high-grade (stage III and IV) tumors compared with that in low-grade (stage I and II) tumors, and CRC patients with high levels of GLI1 or GLI2 showed worse prognoses (Supplementary Fig. 16). The gene discussed is GLI2; the disease is colorectal carcinoma.