Ripk3+/+ to Ripk3+/+ chimeric mice developed renal failure and serious tubular injury as determined by PAS staining at 2 and 14 days after IRI, but those characteristic symptoms were significantly reduced in Ripk3+/+ to Ripk3−/− and Ripk3−/− to Ripk3−/− chimeras (Fig. 5a–c). This evidence concerns the gene RIPK3 and Renal insufficiency.