To further investigate whether Ripk3 and Mlkl deficiency could still reduce renal fibrosis progression after the similar initial renal injury, WT mice group were subjected to renal pedicle clamping for 35 min before reperfusion; whereas Ripk3−/−, Mlkl−/− and Ripk3−/−Mlkl−/− groups were subjected to 43-min renal ischemia. This evidence concerns the gene RIPK3 and renal fibrosis.