By chemokine profiling, we validated CXCL1 as the most abundant secretor released from TAMs, and CXCL1 administration promoted breast cancer metastasis via the nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB)/sex determining region Y-box 4 (SOX4) signaling in both murine and human models. The gene discussed is NFKB1; the disease is breast carcinoma.