To further pursue the association of NUAK2 with tumor grade, we further analyzed cell lines established from HG-MI (TCCSUP and T24; grade 3 or 4), HG-NMI (RT112: grade 2) or LG (SW780 and HTB-2; grade 1) bladder cancer patient tumors42. This evidence concerns the gene NUAK2 and urinary bladder cancer.