RA-FLS (n = 4–6) were stimulated with recombinant tumor necrosis factor-α (TNF-α) (10 ng/ml) and interleukin-1β (IL-1 β) (1 ng/ml) or interleukin 6 (IL-6) (100 ng/ml) with soluble IL-6 receptor (sIL-6R) (100 ng/ml) for 24 h to explore the effects on miR-218 expression. The gene discussed is TNF; the disease is rheumatoid arthritis.