These results suggest that miR-218 promote osteogenic differentiation of RA-FLS through ROBO1 suppression and inhibition of DKK1 secretion, therefore activation of Wnt/β-catenin signaling is presumed to be the possible mechanism of miR-218-induced osteogenesis of RA-FLS. The gene discussed is ROBO1; the disease is rheumatoid arthritis.