To rule out the possibility that the deficiency in the hematopoietic reconstitution of HSCs is caused by the long-term suppression from leukemic cells, we injected normal LSK-tomato+ cells into Ctrl+A2D and B-ALL+A2D mice and then sorted the tomato+ cells from the chemotherapy-induced BM with flow cytometry in 72 h. Here, CTRL is linked to acute lymphoblastic leukemia.