To investigate the effects of TREM2 variants on microglial function, iPSC-MGLCs were generated from a patient with NHD caused by a homozygous TREM2 T66M variant, two unaffected relatives carrying a heterozygous TREM2 T66M variant, and one NHD patient carrying a homozygous TREM2 W50C variant (Table 1; Dardiotis et al., 2017, Guerreiro et al., 2013b). Here, TREM2 is linked to Nasu-Hakola disease.