We focused on neuronal cells, specifically investigating the dissociation between Tau and the microtubulin network in an in vitro model of autophagy dysfunction, mimicking a key pathology in Alzheimer’s disease, by analyzing the change in colocalization between Tau and acetylated α-tubulin at a control, 6 hour and 24 hour time points. This evidence concerns the gene MAPT and early-onset autosomal dominant Alzheimer disease.