The results showed that only aldehyde dehydrogenase 2 (ALDH2), neural precursor cell expressed, developmentally down-regulated 9 (NEDD9), filamin A (FLNA), lamin B receptor (LBR), twinfilin actin binding protein 1 (TWF1), and serine and arginine rich splicing factor 1 (SRSF1) were independent genes that associated with the OS of colon cancer patients, with β values of −1.343, −0.051, 0.492, −0.020, −0.181, and −1.938, respectively. This evidence concerns the gene SRSF1 and colonic neoplasm.