Additionally, expression of HopZ1a from the plasmid protected bean against infection by Pph 1448A, since leaves inoculated with 5 × 105 cfu/ml of Pph 1448A carrying pHopZ1a displayed no disease symptoms 7 dpi, looking like those inoculated with a ΔhrcV T3SS-defective mutant, while those inoculated with Pph 1448A or Pph 1448A expressing HopZ1aC216A were fully symptomatic (Figure 1D). The gene discussed is ENO1; the disease is infection.