In addition, real-time PCR and western blot for rescue experiments showed that miR-485-5p negatively regulated the expression of FZD7, the accumulation of both cytoplasmic and nuclear β-catenin, and the expression of Wnt/β-catenin pathway downstream targets c-Myc and cyclin D1 in HCC cells. The gene discussed is MYC; the disease is hepatocellular carcinoma.