TWIST1 and hepatocellular carcinoma: To elucidate the potential regulatory mechanisms of TWIST1 signaling in tumorigenesis and metastasis, co-immunoprecipitation coupled with two-dimensional gel electrophoresis (2-DE) and liquid chromatography–mass spectrometry was conducted to identify TWIST1-interacting proteins in lysates of the aggressive hepatoma cell line SK-Hep1 (Fig. 1a).