To further determine the role of JAZF1 on hepatic steatosis, Adenovirus expressing JAZF1 (Ad-JAZF1) and small hairpin RNA directed against the coding region of JAZF1 (Ad-shJAZF1) were constructed for up- or down-regulation of JAZF1 in HepG2 cells and Mouse primary hepatocytes (MPHs). This evidence concerns the gene JAZF1 and fatty liver disease.