However, HA-ARD1 and its nonphosphorylatable mutant HA-ARD1 S209A restrained the phosphorylation levels of S6K1, while the phosphorylation-mimic mutant HA-ARD1 S209E increased S6K1 phosphorylation in TNFα-treated cancer cells (Fig. 2b). The gene discussed is NAA10; the disease is cancer.