SFRP2 and neoplasm: Figure 3 shows that it clusters with RASSF1A, RARβ, and SFRP2. Although TWIST1 is considered a master regulator of EMT, DNA methylation may not be the main mechanism that regulates TWIST1 expression in mesenchymal cells. Indeed, it has been shown that TWIST1 methylation did not correlate with either mRNA or protein expression in tumours [41].