Chemotherapy treatment increased the level of autophagosomes (yellow dots in merged images) in the control group, while the level of autophagosome-lysosomes (red dots in merged images after the GFP quenched) were significantly increased after overexpression of HSP90AA1, meaning that HSP90AA1 is able to promote the autophagic flux in chemotherapy agents treated osteosarcoma cells (Fig. 3b). This evidence concerns the gene HSP90AA1 and osteosarcoma.