The Shh signalling pathway affects the EMT, especially during embryonic development and during metastasis in various cancers.28, 30 Self‐renewals in pancreatic cancer stem cells decreased with inhibition of the Shh signalling pathway by sulphoraphane.32 Our results show that certain EMT genes (N‐cad, α‐Sma, vimentin, fibronectin and Tgf‐β1) increased during sphere formation and propagation when Shh was activated by Pur. The gene discussed is ACTA1; the disease is pancreatic neoplasm.