In addition, while ethanolic twig extract shows cytotoxic activity against ECV-304 cancer cells in vitro [96], methanolic extracts of the leaves, pericarp, or seeds of A. muricata all exert toxicity against glioma U87MG, breast MDA-MB-231-pcDNA3 and MDA-MB-231-BCRP clone 23, colorectal HCT-116 (p53+/+), and HCT-116 (p53−/−) cancer cells [24]. The gene discussed is TP53; the disease is central nervous system cancer.