To confirm that the protective effect of hyperglycemia is independent of the intracellular amount of the chemotherapeutics, we observed that in HT29 cells the amount of intracellular of rhodamine 123, taken as an index of Pgp plus MRP activity, and Hoechst 33342, taken as an index of BCRP activity (Figure 7A), and MRP1, 5 and 8, BCRP and Pgp gene expression (Figure 7B) were not modified by HG incubation. This evidence concerns the gene PGP and Hyperglycemia.