Immunoblot analysis confirmed these glioma-CM-induced changes in FSP-1 and VEGFR-2 expression, and also showed that glioma-CM induced expression of alpha-smooth muscle actin (α-SMA), another mesenchymal marker protein (Fig. 2c), consistent with our previous work showing that glioma-CM induced mesenchymal transformation in ECs14. Here, ACTA1 is linked to glioma.