Immunoblotting using the penta-his antibody detected the “octa-His” tag from insect cell expressing Nicastrin (Fig. 3I; lane 4) and not endogenous Nicastrin from human neuroblastoma SHSY5Y cells (Fig. 3I; lane 3), while the Nicastrin antibody detected NCT in both the SHSY5Y (Fig. 3I; lane 1) and PS1(wt)-γ-secretase expressing insect cells (Fig. 3I; lane 2). This evidence concerns the gene NCSTN and neuroblastoma.