Indeed, we observed increased numbers of CD160+ PD-1+ CD8+ T cells (Fig. 6f) and CD160+ CD4+ T cells (Fig. 6g) in co-cultures with HIV-infected MDMs, which was not observed when HIV-1 infection in MDMs was inhibited (AZT), soluble IFN-I was neutralized (B18R), or when MDMs were infected with HIV-1/Rev-M10 mutant, which displays a block to HIV-1 icRNA nuclear export (M10). This evidence concerns the gene CD4 and HIV-1 infection.